Coding

Part:BBa_J24807:Design

Designed by: Neil Parikh   Group: iGEM06_Brown   (2008-03-19)


hypothetical activator/repressor of Mer operon


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 78


Design Notes

We added in extra nucleotides that are complementary to the upstream and downstream regions on the plasmid to enable PCR reactions to work.


Source

This part is protein coding. It comes from plasmid pMOL28 in Ralstonia metallidurans.

Link: http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?val=56550557&from=145400&to=145834&view=gbwithparts

We have added a primer that is upstream and compatible with the BioBrick Standard Assembly format.

References